Composite

Part:BBa_K4164009

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


constitutive expression of FXR-ddRFPA1

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried low intensity promoter at first. This composite part contains the low intensity promoter (BBa_J23105) and RBS. The composite part can be directly imported into plasmid and express FXR-ddRFPA1. If you want to learn about the detailed introduction of FXR-ddRFPA1, you can click the link below. https://parts.igem.org/Part:BBa_K4164994

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1533
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1122


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Parameters
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